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KMID : 0545120110210111101
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 11 p.1101 ~ p.1108
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Evaluation of the Coal-Degrading Ability of Rhizobium and Chelatococcus Strains Isolated from the Formation Water of an Indian Coal Bed
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Durgesh Narain Singh
Anil Kumar Tripathi
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Abstract
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The rise in global energy demand has prompted researches on developing strategies for transforming coal into a cleaner fuel. This requires isolation of microbes with the capability to degrade complex coal into simpler substrates to support methanogenesis in the coal beds. In this study, aerobic bacteria were isolated from an Indian coal bed that can solubilize and utilize coal as the sole source of carbon. The six bacterial isolates capable of growing on coal agar medium were identified on the basis of their 16S rRNA gene sequences, which clustered into two groups; Group I isolates belonged to the genus Rhizobium, whereas Group II isolates were identified as Chelatococcus species. Out of the 4 methods of whole genome fingerprinting (ERIC-PCR, REP-PCR, BOX-PCR, and RAPD), REPPCR showed maximum differentiation among strains within each group. Only Chelatococcus strains showed the ability to solubilize and utilize coal as the sole source of carbon. On the basis of 16S rRNA gene sequence and the ability to utilize different carbon sources, the Chelatococcus strains showed maximum similarity to C. daeguensis. This is the first report showing occurrence of Rhizobium and Chelatococcus strains in an Indian coal bed, and the ability of Chelatococcus isolates to solubilize and utilize coal as a sole source of carbon for their growth.
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KEYWORD
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Coal bed, Coal solubilisation, rep-PCR, 16S rRNA gene sequence, Chelatococcus
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